Free radical scavenging activity of type II collagen peptides and chondroitin sulfate oligosaccharides from by-products of mottled skate processing

The by-products of the skate processing industry have bioactive properties but are mostly discarded. In present study, cartilage, a major by-product that is rich in chondroitin sulfate (CS) and type II collagen were investigated. Enzymatically-hydrolyzed peptides (CP) thermally hydrolyzed CS oligosaccharides (CSo) from cartilage prepared their antioxidant activities studied. High free radical scavenging activity was observed <3 kDa MW fraction papain CP (Pa-CP). CSo also had high activity, mainly due to intermediate final products non-enzymatic browning reaction resulting high-temperature hydrolysis. These may included flavonoid-like compounds. Furthermore, Pa-CP not cytotoxic. Thus, derived be beneficial ingredients. study contribute development functional health foods achieving zero-waste processing.